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Accueil > Seminars > Archives > Archives 2008

Imaging and quantification of nano-layers in water with the SEEC technique

Olivier Theodoly, Marie-Pierre Valignat & Sylvain Gabriele, Adhésion cellulaire et Inflammation INSERM U600 - CNRS UMR6212

published on , updated on

The SEEC technique (surface enhanced ellipsometric technique) permits to
image objects of nanometric thickness with a conventional microscope. We
present here recent developments of the SEEC technique for applications
in an immersed environment. New reflecting and transparent SEEC
substrates have been fabricated, a calibration protocol has been
developped to allow absolute thickness measurements, and the coupling of
the SEEC technique with microfluidics devices has been achieved, which
opens the possibility to perform high-throughput measurements. We
present results with silane monolayers, vesicles interacting with
surfaces, living cells, nanoparticle suspensions and polyelectrolytes
multilayers.

- Work supported by Rhodia and Nanolane companies.

- Site du laboratoire:
http://www.marseille.inserm.fr/u600